2017-8-11 16:0:32025-06-16 23:36:44
_ec
locus
BSU07430
BSU_07430
geneLength
1128
1131
outlinks
bsu
BSU07430
BSU_07430
Gene
Coordinates
816,113 → 817,243
816,113 817,243
Gene
Phenotypes of a mutant
poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]
poor growth at low temperatures (16 to 20C) [Pubmed|23175651]
The protein
Catalyzed reaction/ biological activity
RNA helicase
required for [SW|ribosome] assembly [Pubmed|23175651]
RNA helicase
required for [SW|ribosome] assembly [Pubmed|23175651]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
The protein
Protein family
helicase C-terminal domain (according to Swiss-Prot) [SW|DEAD-box RNA helicases]
[SW|helicase family] (according to UniProt)
[SW|DEAD-box RNA helicases] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|CshA]], [[protein|CshB]], [[protein|DeaD]]
[[this]]
Biological materials
Mutant
MGNA-C239 ([[gene|yfmL]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2237 NBRP B. subtilis, Japan]
GP1053 (Δ[[gene|yfmL]]::erm), available in the [SW|Jörg Stülke]'s lab [pubmed|23175651]
MGNA-C239 ([[gene|yfmL]]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2237 NBRP B. subtilis, Japan]
GP1053 ([[gene|yfmL]]::erm), available in the [SW|Jörg Stülke]'s lab [pubmed|23175651]
BKE07430 ([[gene|yfmL]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCTCCTCAATTC, downstream forward: _UP4_TAAAAAGAAGGGCGAAGAGC
BKK07430 ([[gene|yfmL]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCTCCTCAATTC, downstream forward: _UP4_TAAAAAGAAGGGCGAAGAGC
Biological materials
Expression vector
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1069 (spc), available in [SW|Jörg Stülke]'s lab
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|20572937]
Biological materials
FLAG-tag construct
GP1066 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s
GP1066 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
References
The protein
[SW|Domains]
[SW|Helicase ATP-binding domain] (aa 35-205) (according to UniProt)
[SW|Helicase C-terminal domain] (aa 231-374) (according to UniProt)
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1069 (spc), available in [SW|Jörg Stülke]'s lab